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reported the kinetics of the two different GDH forms from the bovine brain (bGDH1 and bGDH2) ( GDH in brain development Since glutamate is the primary excitatory neurotransmitter in the central nervous system (CNS), mitochondrial function and glutamate metabolism are closely related in the brain ( Target neurons are depolarized by glutamate produced by active synapses via certain receptors (Spreafico et al., 1994)

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Briefly, cells treated as above were added to 24-well plates and incubated for 20 min with DCFH-DA (10 mol/L) at 37 C